Rapid Histone Extraction for Electrophoretic Analysis

Rapid histone extraction for electrophoretic analysis.

with 5% acetic acid and, after 10 min, the gel was finally washed in distilled water and air-dried onto the glass support. Air-drying allows the long and thin gel to be easily submitted to photographic and/or digital recording using a sequencing gel-sized scanner, such as the HP ScanJet 4C/T (Hewlett-Packard, Palo Alto, CA, USA). This avoids wethandling or the need of an apparatus for gel-dryin...

Rapid preparation of affinity-purified lipopolysaccharide samples for electrophoretic analysis.

Lipopolysaccharides (LPS) are the major components of the outer membrane of gram-negative bacteria. These surface molecules are relevant both for the outer membrane stability and for the interaction of the bacteria with other organisms and with the environment (18). Extensive literature is available concerning LPS physiology in different symbiotic (parasitic) and pathogenic host-bacterial inter...

A Simple and Rapid Leaf Genomic DNA Extraction Method for Polymerase Chain Reaction Analysis

In plants, secondary metabolites and polysaccharides interfere with genomic isolation procedures and downstream reactions such as restriction enzyme analysis and gene amplification. The removal of such contaminants needs complicated and time-consuming protocols. In this study, a simple, rapid and efficient method for leaf DNA extraction was optimized. This method use small amount of plant mater...

Rapid, efficient procedure for extraction of drugs for toxicological analysis.

In most cases isolation of drugs from biological samples taken from drug overdose cases has been performed by solvent extraction, for example at pH 9.5 (1), pH 7 (2), or without pH adjustments (3). These techniques do not efficiently extract both strong bases (such as amphetamine) and strong acids (such as acetylsalicylic acid). A two-step extraction of the biological sample at different pH val...

Evaluation of extraction chromatography resins for rapid actinide analysis